Both the filtrate and the dialyzate of agave pollen can activate the enzyme, thus proving that there was activating substance of the thiol group in agave pollen.
The glomerular filtrate is not the same consistency as urine, as much of it is reabsorbed into the blood as the filtrate passes through the tubules of the nephron.
Sometimes fluid or electrolytes can move back from the filtrate into the blood - called reabsorption, and sometimes more fluid or electrolytes can move from the blood to the fitrate - called secretion.
Blood gets into the kidney through the renal artery, into tiny clumps of arterioles called glomeruli where it's initially filtered, with the filtrate, the stuff filtered out, moving into the renal tubule.
In these situations, the nephron tries to hold on to salt and water, so by the time filtrate has moved into the distal tubule, there's very little of both sodium and water remaining in the lumen.