So the absorbing wavelength is selected,the operating condition optimized,and the quantitative and qualitative determination method on Polyoxin B by HPLC-DAD established.
Well equipped, these instruments feature: multiple entrance and exit ports, high precision wavelength drives, extended range operation, Echelle, and oversize grating mounting capabilities.
The wavelength division effect of grism in a multi-wavelengths optical system is discussed, the grism consist of a tranditional optical prism and a diffraction optical phase grating.