Taq polymerase is used to amplify the cDNA through denaturation, annealing and extension steps.

Taq聚酶通过变性、退火、延伸等用于扩增cDNA。

Once we've got the cDNA, the steps of RT-PCR are pretty much the same as PCR.

一旦我们获得了互补DNA，RT-PCR之后的和PCR相似。

If the viral RNA is present, the primers attach to the RNA strand and then reverse transcriptase synthesizes a cDNA strand.

如果存在相关病毒RNA，引物会与RNA单链结，之后逆转酶将会成一条互补DNA链。

Julius, he started with a cDNA library, it's called, it's copies out of all proteins that are synthesized in a cell.

利叶斯从 cDNA 文库开始，它是从细胞中成的所有蛋白质中复制出来的。

Initially, production in bacteria was the only route available and cDNA clones were used as they contained no introns.

cDNA需要与原核生物的转和翻译信号相连，并且需要插入多拷贝的质粒。

RNA is complimentary to DNA though, so if an RNA strand has a nucleotide sequence 3' - A A G U C C A G U - 5', then the complimentary DNA or cDNA would be 5' - T T C A G G T C A - 3'.

但RNA与DNA之间互补，所以如果一条RNA链拥有如下核苷酸序列：3' - A A G U C C A G U - 5'，那么与之互补的DNA是5' - T T C A G G T C A - 3'。