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We have cloned a novel human gene CRALBPL (cellular retinaldehyde-binding protein-like) containing SEC14 domain from the cDNA library of human fetal brain.

我们从人胎脑cDNA文库一条含有SEC14结构域的人类新基因类细视黄醛结合蛋白(CRALBPL)。

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Zoa was first isolated from Z. officinale, which belongs to Zingiberaceae family.The full-length cDNA of zoa was 768bp with an opening reading frame of 510bp encoding a protein with 169 amino acids.

首次从姜科植物姜了姜凝集素基因 zoa, 姜凝集素基因的全长为768bp, 含有一个510bp的开放阅读框,编码169个氨基酸。

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Osmosis-遗传

Taq polymerase is used to amplify the cDNA through denaturation, annealing and extension steps.

Taq聚合酶通过变性、退火、延伸等步用于扩增cDNA

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Osmosis-遗传

If the viral RNA is present, the primers attach to the RNA strand and then reverse transcriptase synthesizes a cDNA strand.

如果存在相关病毒RNA,引物便会RNA结合,之后逆转录酶将会合成一条互补DNA

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Osmosis-遗传

Once we've got the cDNA, the steps of RT-PCR are pretty much the same as PCR.

一旦我们获得了互补DNART-PCR之后的步便和PCR相似。

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2021诺贝尔得主访谈录

Julius, he started with a cDNA library, it's called, it's copies out of all proteins that are synthesized in a cell.

朱利叶斯从 cDNA 文库开始,它是从细胞中合成的所有中复制出来的。

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Research articles

This antisense cDNA, it's like the mirror image of that code.

这个反义cDNA, 就像是那个代码的镜像。

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2017级专外有声

Initially, production in bacteria was the only route available and cDNA clones were used as they contained no introns.

cDNA需要原核生物的转录和翻译信号相连,并且需要插入多拷贝的粒。

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Research articles

So this antisense cDNA, it binds to the gene's RNA message and that basically blocks the cell from being able to produce the protein it was supposed to make.

所以这个反义 cDNA 基因的 RNA 信息结合,基本上阻止了细胞能够产生它应该制造的

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Osmosis-遗传

RNA is complimentary to DNA though, so if an RNA strand has a nucleotide sequence 3' - A A G U C C A G U - 5', then the complimentary DNA or cDNA would be 5' - T T C A G G T C A - 3'.

但RNADNA之间互补,所以如果一条RNA拥有如下核苷酸序列:3' - A A G U C C A G U - 5',那么之互补的DNA便是5' - T T C A G G T C A - 3'。

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