Any blood vessels encountered along the way are ligated.

操作过程中遇到任何血管都要结扎。

Open, identify, ligate, remove, irrigate, close.

打开，确认，结扎，移，，缝合。

Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功筛选。

They found that it wasn't necessary to ligate a dog's pancreatic ducts in order to collect insulin.

他们发现没有必要结扎狗胰管来收集胰岛素。

It read, " Ligate pancreatic ducts of dogs." Banting's idea was to close off the small passages in a dog's pancreas that release its products into the rest of its body.

上面写着：“结扎狗胰管。”班廷想法是关闭狗胰腺中小通道，这些通道将其产物释放到身体其他部位。