sciatic

Objective:To study the influence of ethanol on absolute refractory period of sciatic nerve in hoptoad.

探讨乙醇神经干应期的影响。

We used ethanol at different concentrations for the sciatic nerve stem of the unorganized hoptoad and measures its absolute refractory period.

本实验离体蟾蜍坐骨神经干进行同浓度乙醇处理,测其应期。

Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.

先用IV型胶原酶和胰蛋白酶消化出生3天的SD鼠臂层神经和坐骨神经织块后再将织块贴壁培养。